Journal
DEVELOPMENT
Volume 136, Issue 11, Pages 1939-1949Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.033803
Keywords
Apc; Wnt; beta-catenin; Fgf8; Msx1; Stem cells; Tooth regeneration
Categories
Funding
- NIDCR [5RL1DE019021, 5R37DE011697]
- NIH Initiative for Roadmap for Medical Research to R.L.M. [8UL1DE019581]
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [R37DE011697, UL1DE019581, RL1DE019021] Funding Source: NIH RePORTER
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The ablation of Apc function or the constitutive activation of beta-catenin in embryonic mouse oral epithelium results in supernumerary tooth formation, but the underlying mechanisms and whether adult tissues retain this potential are unknown. Here we show that supernumerary teeth can form from multiple regions of the jaw and that they are properly mineralized, vascularized, innervated and can start to form roots. Even adult dental tissues can form new teeth in response to either epithelial Apc loss-of-function or beta-catenin activation, and the effect of Apc deficiency is mediated by beta-catenin. The formation of supernumerary teeth via Apc loss-of-function is non-cell-autonomous. A small number of Apc-deficient cells is sufficient to induce surrounding wild-type epithelial and mesenchymal cells to participate in the formation of new teeth. Strikingly, Msx1, which is necessary for endogenous tooth development, is dispensable for supernumerary tooth formation. In addition, we identify Fgf8, a known tooth initiation marker, as a direct target of Wnt/beta-catenin signaling. These studies identify key mechanistic features responsible for supernumerary tooth formation.
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