Journal
DEVELOPMENT
Volume 135, Issue 23, Pages 3829-3838Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.029009
Keywords
Drosophila; Microtubules; PAR proteins; PIP2; PIP5K; Polarity
Categories
Funding
- CNRS, Association pour la recherche sur le cancer [4446, 3297]
- ACI 'Jeune chercheur' programme of the Ministere de la Recherche, ANR Blanche [Blan06-3-139786]
- ICREA Funding Source: Custom
Ask authors/readers for more resources
The attachment of the cytoskeleton to the plasma membrane is crucial in controlling the polarized transport of cell-fate-determining molecules. Attachment involves adaptor molecules, which have the capacity to bind to both the plasma membrane and elements of the cytoskeleton, such as microtubules and actin filaments. Using the Drosophila oocyte as a model system, we show that the type I phosphatidylinositol 4-phosphate 5-kinase (PIP5K), Skittles, is necessary to sustain the organization of microtubules and actin cytoskeleton required for the asymmetric transport of oskar, bicoid and gurken mRNAs and thereby controls the establishment of cell polarity. We show that Skittles function is crucial to synthesize and maintain phosphatidylinositol 4,5 bisphosphate (PIP2) at the plasma membrane in the oocyte. Reduction of Skittles activity impairs activation at the plasma membrane of Moesin, a member of the ERM family known to link the plasma membrane to the actin-based cytoskeleton. Furthermore, we provide evidence that Skittles, by controlling the localization of Bazooka, Par-1 and Lgl, but not Lkb1, to the cell membrane, regulates PAR polarity proteins and the maintenance of specific cortical domains along the anteroposterior axis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available