Journal
DEVELOPMENT
Volume 135, Issue 8, Pages 1415-1425Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.019497
Keywords
mouse; primordial germ cells; sertoli cell; membrane trafficking; sdmg1 (Tmem184a)
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Funding
- Medical Research Council [G120/952, MC_U127580973] Funding Source: researchfish
- MRC [MC_U127580973, G120/952] Funding Source: UKRI
- Medical Research Council [G120/952, U.1275.01.008(80973), MC_U127580973, MC_PC_U127580973] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
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In mammals, the supporting cell lineage in an embryonic gonad communicates the sex-determining decision to various sexually dimorphic cell types in the developing embryo, including the germ cells. However, the molecular nature of the sex-determining signals that pass from the supporting cells to the germ cells is not well understood. We have identified a conserved transmembrane protein, Sdmg1, owing to its male-specific expression in mouse embryonic gonads. Sdmg1 is expressed in the Sertoli cells of embryonic testes from 12.5 dpc, and in granulosa cells of growing follicles in adult ovaries. In Sertoli cells, Sdmg1 is localised to endosomes, and knock-down of Sdmg1 in Sertoli cell lines causes mis-localisation of the secretory SNARE Stx2 and defects in membrane trafficking. Upregulation of Sdmg1 appears to be part of a larger programme of changes to membrane trafficking pathways in embryonic Sertoli cells, and perturbing secretion in male embryonic gonads in organ culture causes male-to-female germ cell sex reversal. These data suggest that changes that occur in the cell biology of embryonic Sertoli cells may facilitate the communication of male sex-determining decisions to the germ cells during embryonic development.
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