Journal
JOURNAL OF ORAL PATHOLOGY & MEDICINE
Volume 45, Issue 2, Pages 111-118Publisher
WILEY
DOI: 10.1111/jop.12331
Keywords
invasion; migration; PRL-3; prognosis; salivary adenoid cystic carcinoma
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Funding
- National Nature Science Foundation of China [NSFC81472228, NSFC81272953]
- Guangdong Natural Science Foundation [S2012010008665]
- Ministry of Education [20120171110050]
- Program for New Century Excellent Talents in University [NCET-10-0857]
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BackgroundPRL-3 had been found to be involved in tumorigenesis in various malignancies. In this study, we investigated the role of PRL-3 in the development, migration, and invasion of salivary adenoid cystic carcinoma (SACC). MethodsImmunohistochemistry (IHC) was used to analyze the role of PRL-3 in the development and prognosis of SACC. Then, we overexpressed or inhibited the expression of PRL-3 in paired SACC cells to analyze the role of PRL-3 in the migration and invasion of SACC. In vitro migration and invasion assays were used. Western blotting was used to detect metastasis-related protein levels. ResultsIHC results confirmed that the deregulation of PRL-3 was a frequent event in SACC; the upregulation of PRL-3 was related to clinical stages, vital status, and distant metastasis, which was associated with reduced overall survival and disease-free survival. SACC-LM cells with higher migratory and invasive abilities had more robust PRL-3 protein expression than SACC-83 cells with lower migratory and invasive abilities. PRL-3 overexpression promoted cell migration, invasion, and proliferation, led to simultaneous upregulation of phosphorylated PRL-3, pERK1/2, Slug, vimentin, and downregulation of E-cadherin in SACC-83 cells. However, the inhibition of PRL-3 by PRL-3 inhibitor or PRL-3 siRNA in SACC-LM cells inhibited cell migration, invasion, and proliferation, resulted in simultaneous downregulation of phosphorylated PRL-3, pERK1/2, Slug, vimentin, and upregulation of E-cadherin. ConclusionsOur results confirm that PRL-3 plays an important role in the development of SACC and contributes to the migratory and invasive abilities of SACC.
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