Journal
DESALINATION
Volume 250, Issue 3, Pages 1125-1127Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.desal.2009.09.123
Keywords
Autographa californica; M nucleopolyhedrovirus; Membrane filtration; Virus concentration; Downstream processing
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Concentration and retention of a rod-shaped virus during tangential flow ultrafiltration (UF) was assessed to evaluate the potential of membrane-based downstream methods with advantages such as easy scale-up for industrial processes. A recombinant baculovirus of the non-spherical Autographa californica M nucleopolyhedrovirus (AcMNPV), vHSGFP, expressing egfp was filtered using polyethersulfone membranes ranging from 30 to 1000 kDa molecular weight cut-off (MWCO). A 20-fold virus concentration was achieved when a membrane cut-off range of 100 to 1000 kDa was tested. Fouling was observed and cake formation and pore plugging were postulated as concurrent causes with different impact depending on the MWCO. A reduction of virus concentration in the range of 2 to 5 log units in the permeate was observed illustrating the potential of membrane-based virus filtration as a useful unit operation in downstream processing. (C) 2009 Elsevier B.V. All rights reserved.
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