Journal
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
Volume 41, Issue 9, Pages 1399-1405Publisher
WILEY-BLACKWELL
DOI: 10.1111/jog.12737
Keywords
aquaporin 1; cell culture; maternal-fetal fluid balance; trophoblast; water permeability
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Funding
- National Natural Science Foundation [30973206, 81170594]
- National Distinguished Young Scholars Foundation [30325011]
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Aim: Aquaporins (AQP) are water channel proteins, and some play an important role in maternal-fetal fluid exchange. The present study aimed to measure the osmotic water permeability in primary cultures of trophoblast cells from AQP1-deficient (AQP1(-/-)) pregnant mice and to define the quantitative role of AQP1 in water transport across the trophoblast plasma membrane. Material and Methods: Trophoblast cells were obtained from placental tissue cell culture of AQP1(-/-) pregnant mice and were characterized by cytokeratin 7 immunostaining. The expression of the AQP1 gene in trophoblast cells of wild-type (AQP1(+/+)) mice was confirmed by immunofluorescence. The osmotic water permeability of trophoblast plasma membranes was measured by a calcein fluorescence quenching method in response to osmotic gradients. Results: A primary cell culture system for trophoblasts was successfully established. Immunofluorescence showed the expression of AQP1 in the trophoblast cell membrane of AQP1(+/+) mice. The osmotic water permeability of AQP1(-/-) trophoblast cells was significantly lower than that in AQP1(+/+) trophoblast cells, in response to both hypotonic and hypertonic challenges. Conclusion: The results suggest an important role of AQP1-mediated plasma membrane water permeability in maternal-fetal fluid balance and also provide a potential direction for the identification of therapeutic targets for the treatment of abnormalities in amniotic fluid volume.
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