Journal
DENTAL MATERIALS JOURNAL
Volume 27, Issue 5, Pages 744-750Publisher
JAPANESE SOC DENTAL MATERIALS DEVICES
DOI: 10.4012/dmj.27.744
Keywords
Osteoblast; RGD peptide; GeneChip
Funding
- Japan Society for the Promotion of Science [A1-16209063, C-19592260]
- Ministry of Education, Culture, Sports, Science and Technology
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Enhanced adhesion and migration of osteoblastic cells on titanium (Ti) surface is believed to increase the success rate of implant therapy. A GRGDSP peptide derived from fibronectin was coated on Ti surfaces using a tresyl chloride activation technique, and then MC3T3-E1 osteoblastic cells were cultured on the Ti surfaces. After 15 days, total RNA was isolated from the cells and gene expression level were analyzed by Affymetrix GeneChip system. The expression levels of many genes in MC3T3-E1 cells cultured on GRGDSP-coated Ti surface were altered when compared to uncoated Ti. In particular, the elevated mRNA levels of bone sialoprotein (BSP) and osteocalcin (OC) were successfully confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. In light of the results obtained, GRGDSP-coated Ti presented the potential of evolving into a useful biomaterial for successful implant therapy.
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