Development of a functional ruthenium(II) complex for probing hypochlorous acid in living cells

A functional ruthenium(II) complex, [Ru(bpy)(2)(AN-bpy)](PF6)(2) (bpy: 2,2'-bipyridine, AN-bpy: 4-methyl-4'-(4-amino-3-nitro-phenoxy-methylene)-2,2'-bipyridine), has been designed and synthesized as a turn-on luminescent probe for the imaging of hypochlorous acid (HOCl) in living cells. Due to the intramolecular photoinduced electron transfer (PET), the ruthenium(II) complex itself is almost non-luminescent. However, it can specifically and rapidly react with HOCl in aqueous media to afford a highly luminescent derivative, [Ru(bpy)(2)(HM-bpy)](PF6)(2) (HM-bpy: 4-hydroxymethyl-4'-methyl-2,2'-bipyridine), accompanied by a 110-fold luminescence enhancement. Taking advantage of high specificity and sensitivity, and excellent photophysical properties of the ruthenium(II) complex probe, [Ru(bpy)(2)(AN-bpy)](PF6)(2) was successfully applied to the luminescence imaging of the exogenous HOCl in living HeLa cells and the endogenous HOCl in porcine neutrophils. The results corroborate that indeed [Ru(bpy)(2)(AN-bpy)](PF6)(2) is a useful luminescent probe for the monitoring of HOCl in biological systems.