Self-assembled metalla-bowls for selective sensing of multi-carboxylate anions

Two new tetranuclear cationic metalla-bowls 4 and 5 were self-assembled from a bis-pyridine amide ligand (H2L) (1) and arene-ruthenium acceptors, [(Ru-2(mu-eta(4)-C2O4)(eta(6)-p-cymene)(2)](O3SCF3)(2) (2) and [Ru-2(dhnd)(eta(6)-p-cymene)(2)](O3SCF3)(2) (dhnd = 6,11-dihydroxy-5,12-naphthacenedionato) (3), respectively. The metalla-bowls were characterized by multinuclear NMR, ESI-MS, UV-Vis spectroscopy, and single crystal X-ray diffraction study of 4. The crystal structure of 4 reveals unambiguous proof for the molecular shape of the metalla-bowl and the encapsulation of one triflate anion in the cavity through hydrogen bonding. The metalla-bowl 5 has been evaluated for anion binding studies by use of amide ligand as a hydrogen bond donor and arene-Ru acceptor as a signalling unit. UV-Vis titration studies showed that 5 selectively binds with multi-carboxylate anions such as oxalate, tartrate and citrate in a 1 : 1 fashion with high binding constants of 4.0-5.5 x 10(4) M-1. Furthermore, the addition of multi-carboxylate anions into a solution of 5 gave rise to a large enhancement of fluorescence intensity attributable to the blocking of a photo-induced electron transfer process from the arene-ruthenium moiety to the amidic donor in 5. However, the fluorescence intensity almost remains unchanged upon addition of other anions including F-, Cl-, PF6 (-), MeCOO-, NO3- and PhCOO-, as identically seen in the UV-Vis titration experiments, pointing to the high selectivity of 5 for the sensing of multi-carboxylate anions.