Journal
DALTON TRANSACTIONS
Volume 39, Issue 37, Pages 8663-8670Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0dt00137f
Keywords
-
Categories
Funding
- Polish Ministry of Higher Education and Science [KBN N N204 146537]
Ask authors/readers for more resources
The fragment of the zebrafish prion-like protein (PrP-rel-2), encompassing residues 74-86 and unprotected at N-terminus (zf74-86) represents a good model to understand Cu2+ and Zn2+ binding to ligands containing multi-potential metal donor sites. Zf(74-86) contains four His and His-1 N-terminal amine groups which constitute both copper and zinc anchoring sites. The presence of His at the first position additionally provides the histamine-like binding mode which could compete with the multi-His binding mode. In this study the speciation profiles of the Cu2+ and Zn2+ complexes with zf74-86 have been obtained. The main species, dominating at physiological pH, have been fully characterized by using different spectroscopic techniques. The detected NMR chemical shift variations and line broadening enhancements, caused by Zn2+ and Cu2+ respectively, allowed to determine the metal binding sites. Both metal ions showed common binding donor atoms, being 2 or 3 His imidazoles and the N-terminal group involved in Cu2+ and Zn2+ binding.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available