Journal
CYTOTHERAPY
Volume 16, Issue 8, Pages 1117-1120Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.jcyt.2014.02.004
Keywords
metastatic melanoma; rapid expansion; tumor-infiltrating lymphocytes; Wave bioreactor
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Funding
- Italian Foundation for Cancer Research (FIRC)
- Aase and Ejnar Danielsens Foundation
- Danish Cancer Society
- Lundbeck Foundation
- Capital Region of Denmark Research Foundation
- Associazione Italiana per la Ricerca sul Cancro Funding Source: Custom
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Background aims. The high level of complexity of current Good Manufacturing Practice-compliant methods of manufacturing hampers rapid and broad application of treatment with tumor-infiltrating lymphocytes (TILs). Methods. To ensure higher applicability of TIL production to laboratory routine, a practical and simple protocol of TIL manufacturing with the use of a closed-system bioreactor was developed and implemented at our institution. Results. This protocol enabled significant work load reduction during the most labor-intense step of TIL expansion, and allowed generation of high-quality TIL products, which mediated clinical regression in patients with metastatic melanoma. Conclusions. Implementation of simplified methods of TIL expansion will speed up dissemination of TIL methods worldwide and will increase patient access to this highly effective treatment.
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