Journal
CYTOTHERAPY
Volume 13, Issue 3, Pages 341-349Publisher
ELSEVIER SCI LTD
DOI: 10.3109/14653249.2010.520312
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Funding
- Korea government (MEST) [2010-0020265]
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Background aims. Amniotic fluid (AF) is a well-known source of stem cells. However, there have been no reports regarding equine AF stem cells. We have isolated equine AF-derived multipotent stem cells (MSC) (eAF-MSC) and show that these cells exhibit self-renewal ability and multilineage differentiation. Methods. AF was obtained from thoroughbred mares and mononuclear cells (MNC) were isolated by Ficoll--Paque density gradient. We measured the cumulative population doubling level (CPDL) and characterized the immunophenotype by flow cytometry. To investigate differentiation ability, a trilineage differentiation assay was conducted. Results. eAF-MSC could be isolated and the proliferation level was high. eAF-MSC presented typical MSC phenotypic markers, as determined by flow cytometry. Moreover, eAF-MSC showed a trilineage differentiation capability. Conclusions. Equine AF is a good source of MSC. Furthermore, eAF-MSC may be useful as a cell therapy application for horses.
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