Journal
CYTOTHERAPY
Volume 12, Issue 7, Pages 870-880Publisher
ELSEVIER SCI LTD
DOI: 10.3109/14653249.2010.501789
Keywords
cytokines; growth factors; mesenchymal stromal cells; tissue regeneration
Categories
Funding
- EC [LSHB-CT-2005-518233]
- Interdisciplinary Centre for Clinical Research within the Faculty of Medicine at the RWTH Aachen University [TVB 111]
Ask authors/readers for more resources
Background aims. The beneficial effect of human (h) mesenchymal stromal cell (MSC) transplantation in a variety of cell-based intervention strategies is widely believed to be because of paracrine mechanisms. The modification of hMSC cytokine and growth-factor expression patterns were studied following exposure to lipopolysaccharide (LPS) and tissue homogenates (representing tissue debris) from normal and pathologic tissues. Methods. Human bone marrow-derived MSC were stimulated with LPS or exposed to homogenate from normal or pathologic rat spinal cord or heart. The expression profiles of a number of cytokines and growth factors were investigated using quantitative reverse transcription (RT)-polymerase chain reaction (PCR) with human-specific primers. The effects of tissue homogenates on hMSC proliferation and migratory behavior were also investigated. Results. Stimulation of hMSC with LPS resulted in an up-regulation of interleukin (IL)-1 beta, IL-6 and IL-8. However, the pattern of up-regulation varied between donor samples. Furthermore, LPS treatment resulted in a donor-dependent alteration of growth factor expression. Induction of a shift in expression pattern was not observed following exposure to homogenates from either normal or pathologic tissues. Tissue homogenates did stimulate cell proliferation, but not migration. Conclusions. The hMSC expression pattern is apparently stable, even when cells are confronted by debris from different tissue types. However, treatment of hMSC with LPS is able to change the expression of cytokines and growth factors in a donor-dependent manner that may enhance their potential use in regenerative medicine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available