Journal
CYTOSKELETON
Volume 71, Issue 12, Pages 677-694Publisher
WILEY
DOI: 10.1002/cm.21201
Keywords
RhoGTPases; tumor suppressor; GTPase activating protein (GAP); cell dynamics
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Funding
- Ministry of Education Tier 2 Grant [T208A3121]
- Mechanobiology Institute - Singapore National Research Foundation
- Ministry of Education
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Deleted in Liver Cancer-1 (DLC1) is a RhoGTPase-activating protein (GAP) and a tumor suppressor often downregulated in cancers. It is localized to the focal adhesions (FAs) and its absence leads to enhanced cell migration, invasion, and metastasis. Although DLC1 interacts with focal adhesion kinase (FAK), talin, and tensin, its role in focal adhesions dynamics remains unclear. We examined the effect of DLC1 in Human Foreskin Fibroblasts and determined its localization, dynamics and impact on paxillin by Fluorescence Recovery After Photobleaching at both nascent and mature focal adhesions. During early cell spreading, DLC1 is preferentially localized at the inner/mature adhesions whereas phosphorylated paxillin occupies the outer/nascent FAs. In addition, DLC1 downregulates paxillin turnover in a process, that does not require its GAP activity. Instead, it requires the presence of FAK. Acting in concert, both DLC1 and FAK could provide a unique spatio-temporal mechanism to regulate paxillin function in tissue homeostasis. (C) 2014 Wiley Periodicals, Inc.
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