The discontinuous nature of neurofilament transport accommodates both establishment and repair of the axonal neurofilament array

Neurofilaments (NFs) provide structural support to axons. Timely and regional deposition of NFs is essential during axonogenesis, since progressive stabilization of proximal axons is essential to support continued pathfinding of distal axonal regions. NFs undergo short bursts of microtubule-mediated axonal transport interspersed by prolonged pauses. We demonstrate herein that it is this unique on-off method of axonal transport, coupled with the ability of NFs to form cation-dependent, phosphomediated lateral associations that allow neurons to mediate the orderly transition from exploratory process to stabilized axon following synaptogenesis. We further demonstrate how this transport method provides for NF maintenance following maturation and encompasses the potential for regeneration. (c) 2012 Wiley Periodicals, Inc.