Journal
CYTOMETRY PART A
Volume 93A, Issue 10, Pages 987-996Publisher
WILEY
DOI: 10.1002/cyto.a.23589
Keywords
optofluidics; optical microscopy; lab on chip; brightfield; fluorescence
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Last decade's advancements in optofluidics allowed obtaining an ever increasing integration of different functionalities in lab on chip devices to culture, analyze, and manipulate single cells and entire biological specimens. Despite the importance of optical imaging for biological sample monitoring in microfluidics, imaging is traditionally achieved by placing microfluidics channels in standard bench-top optical microscopes. Recently, the development of either integrated optical elements or lensless imaging methods allowed optical imaging techniques to be implemented in lab on chip systems, thus increasing their automation, compactness, and portability. In this review, we discuss known solutions to implement microscopes on chip that exploit different optical methods such as bright-field, phase contrast, holographic, and fluorescence microscopy. (C) 2018 International Society for Advancement of Cytometry
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