Journal
CYTOMETRY PART A
Volume 93A, Issue 12, Pages 1251-1254Publisher
WILEY
DOI: 10.1002/cyto.a.23507
Keywords
circulating tumor cells; metastasis; liquid biopsy; Dean Flow Fractionation; enrichment
Categories
Ask authors/readers for more resources
Circulating tumor cells (CTCs) dissociate from primary tumor into the bloodstream, and carry with them cancer's fingerprints as well as the potential to turn aggressive and metastasize. In order to understand CTCs and develop clinical utility, different methods of enrichment and isolation of CTCs can be used. Here, we report the use of a label-free platform, ClearCell (R) FX which isolates CTCs by their mechanical features and its advantages. The technology utilizes Dean Flow Fractionation (DFF) principle in a spiral microfluidics system to separate the larger CTCs from smaller blood cells. The gentle and fast workflow allows for a range of downstream assays to be performed on the intact CTCs, particularly studies that examine an epithelial cell adhesion molecular (EpCAM)-independent population. Viable, intact cells are also retrievable for development of culture or in vivo models. (c) 2018 International Society for Advancement of Cytometry
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available