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Flow Cytometry Applications for the Analysis of Chemokine Receptor Expression and Function

Journal

CYTOMETRY PART A
Volume 85, Issue 4, Pages 292-301

Publisher

WILEY
DOI: 10.1002/cyto.a.22439

Keywords

flow cytometry; chemokine receptor; antibodies; fluorophores

Funding

  1. Ministero dell'Istruzione dell'Universita e della Ricerca [2002061255, 2007-ENYMAN_003, RBFR08CW8G]
  2. Italian Association for Cancer Research (AIRC)
  3. Fondazione Cariplo
  4. European Community
  5. MRC
  6. Wellcome Trust
  7. MRC [G0901113] Funding Source: UKRI
  8. Chief Scientist Office [ETM/276, ETM/67] Funding Source: researchfish
  9. Medical Research Council [G0901113] Funding Source: researchfish

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Chemokine receptors play an important role in leukocyte migration, both in physiological and pathological conditions, and the interest in new methodologies for their detection is increasing. In this review, we focused on chemokine receptors detection through flow cytometric approaches, including the use of specific antibodies and fluorescent chemokines, and on approaches aimed at the analysis of their functions, from intracellular trafficking to signaling activities. (c) 2014 International Society for Advancement of Cytometry

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