Journal
CYTOMETRY PART A
Volume 83, Issue 9, Pages 767-779Publisher
WILEY
DOI: 10.1002/cyto.a.22295
Keywords
fluorescence microscopy; historical perspective; diffraction-limited microscopy; super-resolution microscopy
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For a little more than a century, fluorescence microscopy has been an essential source of major discoveries in cell biology. Recent developments improved both visualization and quantification by fluorescence microscopy imaging and established a methodology of fluorescence microscopy. By outlining basic principles and their historical development, I seek to provide insight into and understanding of the ever-growing tools of fluorescence microscopy. Thereby, this synopsis may help the interested researcher to choose a fluorescence microscopic method capable of addressing a specific scientific question. (c) 2013 International Society for Advancement of Cytometry
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