Journal
CYTOMETRY PART A
Volume 79A, Issue 4, Pages 317-324Publisher
WILEY-BLACKWELL
DOI: 10.1002/cyto.a.21042
Keywords
flow cytometer; optofluidics; microfluidics; optical detection; CD4; AIDS diagnostics; point of care testing
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We describe a new optical detection technique, termed spatially modulated fluorescence emission, that delivers high signal-to-noise discrimination without precision optics to enable a flow cytometer that can combine high performance, robustness, compactness, low cost, and ease of use. The detection technique is demonstrated with measurements of absolute CD4+ and percentage CD4 counts in human blood. Benchmarking against a commonly used commercial instrument for this test yields excellent agreement for both absolute CD4 and percentage CD4. (C) 2011 International Society for Advancement of Cytometry
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