Journal
CYTOMETRY PART A
Volume 79A, Issue 11, Pages 959-964Publisher
WILEY-BLACKWELL
DOI: 10.1002/cyto.a.21136
Keywords
Plasmodium falciparum; melatonin; cell cycle; flow cytometry
Categories
Funding
- FAPESP [07/52924-0]
- Malaria Pronex
- INCT-INBqMed
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- CAPES
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [07/52924-0] Funding Source: FAPESP
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Melatonin and its derivatives modulate the Plasmodium falciparum and Plasmodium chabaudi cell cycle. Flow cytometry was employed together with the nucleic acid dye YOYO-1 allowing precise discrimination between mono- and multinucleated forms of P. falciparum-infected red blood cell. The use of YOYO-1 permitted excellent discrimination between uninfected and infected red blood cells as well as between early and late parasite stages. Fluorescence intensities of schizont-stage parasites were about 10-fold greater than those of ring-trophozoite form parasites. Melatonin and related indolic compounds including serotonin, N-acetyl-serotonin and tryptamine induced an increase in the percentage of multinucleated forms compared to non-treated control cultures. YOYO-1 staining of infected erythrocyte and subsequent flow cytometry analysis provides a powerful tool in malaria research for screening of bioactive compounds. (C) 2011 International Society for Advancement of Cytometry
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