Journal
CYTOMETRY PART A
Volume 77A, Issue 7, Pages 667-676Publisher
WILEY
DOI: 10.1002/cyto.a.20931
Keywords
NIR; imaging; FRET; FLIM, STED; nanoscale
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Funding
- DFG Research Center Molecular Physiology of the Brain (CMPB)
- Cluster of Excellence Microscopy at the Nanometer Range in Goetungen, Germany
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This review focuses on technical advances in fluorescence microscopy techniques including laser scanning techniques, fluorescence-resonance energy transfer (FRET) microscopy, fluorescence lifetime imaging (FLIM), stimulated emission depletion (STED)-based super-resolution microscopy, scanning confocal endomicroscopes, thin-sheet laser imaging microscopy (TSLIM), and tomographic techniques such as early photon tomography (EPT) as well as on clinical laser-based endoscopic and microscopic techniques We will also discuss the new developments in the field of fluorescent dyes and fluorescent genetic reporters that enable new possibilities in high-resolution and molecular imaging both in in vitro and in vivo. Small animal and tissue imaging benefit from the development of new fluorescent proteins, dyes, and sensing constructs that operate in the far red and near-infrared spectrum. (C) 2010 International Society for Advancement of Cytometry
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