Journal
CYTOMETRY PART A
Volume 75A, Issue 2, Pages 104-113Publisher
WILEY
DOI: 10.1002/cyto.a.20682
Keywords
vibrational spectroscopy; Raman spectroscopy; IR absorption spectroscopy; direct microbial identification; single cell analysis
Categories
Funding
- Deutsche Forschungsgemeinschaft [1257, DE 307/7-1, PO 563/7-1]
- Federal Ministry of Education and Research, Germany (BMBF) [FKZ 13N9549]
Ask authors/readers for more resources
Rapid microbial detection and identification with a high grade of sensitivity and selectivity is a great and challenging issue in many fields, primarily in clinical diagnosis, pharmaceutical, or food processing technology. The tedious and time-consuming processes of current microbiological approaches call for faster ideally on-line identification techniques. The vibrational spectroscopic techniques IR absorption and Raman spectroscopy are noninvasive methods yielding molecular fingerprint information; thus, allowing for a fast and reliable analysis of complex biological systems such as bacterial or yeast cells. In this short review, we discuss recent vibrational spectroscopic advances in microbial identification of yeast and bacterial cells for bulk environment and single-cell analysis. IR absorption spectroscopy enables a bulk analysis whereas micro-Raman-spectroscopy with excitation in the near infrared or visible range has the potential for the analysis of single bacterial and yeast cells. The inherently weak Raman signal can be increased up to several orders of magnitude by applying Raman signal enhancement methods such as UV-resonance Raman spectroscopy with excitation in the deep UV region, surface enhanced Raman scattering, or tip-enhanced Raman scattering. (C) 2008 International Society for Advancement of Cytometry
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available