Journal
CYTOMETRY PART A
Volume 73A, Issue 11, Pages 1043-1049Publisher
WILEY
DOI: 10.1002/cyto.a.20594
Keywords
CD137; 4-IBB; antigen-specific T-cells; adoptive immunotherapy; immune-monitoring
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Funding
- Child Philipp Foundation for Cancer Research, Germany [T237/16586/2007]
- Tour of Hope, Germany
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CD137 (4-1BB) is a member of the TNFR-family with costimulatory function, triggering prosurvival signals in activated T-cells. Upregulation of CD137 upon stimulation allows identifying and isolating live, human antigen-specific CD8+ T-cells of all phenotypes, and therefore provides a comprehensive detection method. Furthermore responses against antigen mixtures can be easily detected, enabling antigen discovery in a stepwise deconvoluting approach. In this article, we will discuss various aspects of this methodology, including potential pitfalls as well as a variety of applications, as illustrated by examples from our laboratory. (C) 2008 International Society for Advancement of Cytometry
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