Journal
CYTOMETRY PART A
Volume 73A, Issue 10, Pages 926-930Publisher
WILEY
DOI: 10.1002/cyto.a.20623
Keywords
immunology; fluorescence-activated cell sorting; knowledge representation
Categories
Funding
- NIAID NIH HHS [N01AI50027, N01AI40076] Funding Source: Medline
- NIA NIH HHS [R01 AG020719] Funding Source: Medline
- NIBIB NIH HHS [EB005034, R01 EB005034, R01 EB005034-04] Funding Source: Medline
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A fundamental tenet of scientific research is that Published results are open to independent validation and refutation. Minimum data standards aid data providers, users, and publishers by providing a specification of what is required to unambiguously interpret experimental;I findings. Here, we present the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard, stating the minimum information required to report flow cytometry (FCM) experiments. We brought together a cross-disciplinary international collaborative group of bioinformaticians, computational statisticians, software developers, instrument manufacturers, and clinical and basic research scientists to develop the standard. The standard was subsequently vetted by the International Society for Advancement of Cytometry (ISAC) Data Standards Task Force, Standards Committee, membership, and Council. The MIFlowCyt standard includes recommendations about descriptions of the specimens and reagents included in the experiment, the configuration of the instrument used to perforin the assays, and the data processing approaches used to interpret the primary Output data. MIFlowCyt has been adopted as a standard by ISAC, representing the FCM scientific community including scientists as well as software and hardware manufacturers. Adoption of MIFlowCYT by the scientific and publishing communities will facilitate third-party Understanding and reuse of FCM data. (C) 2008 International Society for Advancement Cytometry.
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