Journal
CYTOKINE
Volume 61, Issue 1, Pages 245-250Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cyto.2012.10.001
Keywords
IFN-gamma; IL-1 beta; Vitamin D; Pregnancy; Preeclampsia
Funding
- National Council of Science and Technology
- CONACyT, Mexico [106422, 154215]
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Placenta is an important source and target of hormones that contribute to immunological tolerance and maintenance of pregnancy. In preeclampsia (PE), placental calcitriol synthesis is low; whereas proinflammatory cytokines levels are increased, threatening pregnancy outcome. Previously, we showed that calcitriol inhibits Th-1 cytokines under experimental inflammatory conditions in normal trophoblasts. However, a study of the regulation of inflammatory cytokines by calcitriol in trophoblasts from a natural inflammatory condition, such as PE, is still lacking. Therefore, the aim of the present study was to investigate calcitriol effects upon TNF-alpha, IFN-gamma, IL-6 and IL-1 beta in cultured placental cells from pre-eclamptic women by using qPCR and ELISA. Placentas were collected after cesarean section from pre-eclamptic women and enriched trophoblastic preparations were cultured in the absence or presence of different calcitriol concentrations during 24 h. In these cell cultures, pro-inflammatory cytokines TNF-alpha and IL-6 secretion and mRNA expression were downregulated by calcitriol (P < 0.05). No significant effects of calcitriol upon IFN-gamma and IL-1 beta were observed. In addition, basal expression of TNF-alpha, IL-6 and IL-1 beta decreased as the cells formed syncytia. Our study supports an important autocrine/paracrine role of placental calcitriol in controlling adverse immunological responses at the feto-maternal interface, particularly in gestational pathologies associated with exacerbated inflammatory responses such as preeclampsia. (C) 2012 Elsevier Ltd. All rights reserved.
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