Journal
CYTOKINE
Volume 61, Issue 2, Pages 630-638Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cyto.2012.11.014
Keywords
Macrophage; Ba/F3; Bone marrow; Species specificity; Renal
Funding
- BBSRC Case Studentship (BBSRC) at the Roslin Institute and Royal (Dick) School of Veterinary Studies [338BCB R40954]
- Pfizer Animal Health, Kalamazoo, USA
- Biotechnology and Biological Sciences Research Council [BB/G004013/1, BBS/E/D/20251969] Funding Source: researchfish
- Medical Research Council [G0901193] Funding Source: researchfish
- BBSRC [BBS/E/D/20251969, BB/G004013/1] Funding Source: UKRI
- MRC [G0901193] Funding Source: UKRI
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Colony stimulating factor (CSF-1) and its receptor, CSF-1R, have been previously well studied in humans and rodents to dissect the role they play in development of cells of the mononuclear phagocyte system. A second ligand for the CSF-1R, IL-34 has been described in several species. In this study, we have cloned and expressed the feline CSF-1R and examined the responsiveness to CSF-1 and IL-34 from a range of species. The results indicate that pig and human CSF-1 and human IL-34 are equally effective in cats, where both mouse CSF-1 and IL-34 are significantly less active. Recombinant human CSF-1 can be used to generate populations of feline bone marrow and monocyte derived macrophages that can be used to further dissect macrophage-specific gene expression in this species, and to compare it to data derived from mouse, human and pig. These results set the scene for therapeutic use of CSF-1 and IL-34 in cats. (c) 2012 Elsevier Ltd. All rights reserved.
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