Journal
CYTOKINE
Volume 46, Issue 2, Pages 201-210Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cyto.2009.01.006
Keywords
beta-Defensins; Pro-inflammatory cytokines; Oral homeostasis; Keratinocytes; TLRs
Funding
- Pierre Fabre Oral Care
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Gingival tissue faces constant exposure to micro-organisms. It functions as part of the host response, an anti-microbial barrier that recognizes and discriminates between commensal and pathogenic bacteria. This study aimed to evaluate and compare the effects of cell wall extracts from different periodontal bacteria, commensals Streptococcus sanguinis and Fusobacterium nucleatum and the pathogen Porphyromonas gingivalis, on the innate immune response of gingival keratinocytes and the role of TLR2 in regulating this. We assayed mRNA levels to determine the expression of human beta-defensins (h beta D2, h beta D3), interleukin-1 alpha, -1 beta, 6 and 8 and matrix metalloproteinase-9. F. nucleatum extracts induced P-defensin and inflammatory marker mRNA expression at higher levels than A gingivalis. Extracts from the Gram-positive commensal S. sanguinis did not upregulate the host response. TLR2 extinction inhibited the upregulation of p-defensin and cytokine transcripts by F. nucleatum extracts but, in contrast, led to a weak induction of h beta D3 after challenge with S. sanguinis extracts. Although F. nucleatum strongly induces innate immune and inflammatory mediators, S. sanguinis limits their expression through TLR2. Together, our data demonstrate that gingival keratinocytes recognize and discriminate between Gram-positive and Gram-negative commensal extracts, in part through TLR2, to activate different signaling pathways of the innate immune host response. (C) 2009 Elsevier Ltd. All rights reserved.
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