Journal
CYTOGENETIC AND GENOME RESEARCH
Volume 134, Issue 3, Pages 220-228Publisher
KARGER
DOI: 10.1159/000327713
Keywords
CENH3; Centromere; Holocentric chromosome; Luzula elegans; Mitosis; rDNA
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Funding
- IPK (Gatersleben)
- Leibniz Association (WGL)
- Deutsche Forschungsgemeinschaft (DFG)
- LMU
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The structure of holocentric chromosomes was analyzed in mitotic cells of Luzula elegans. Light and scanning electron microscopy observations provided evidence for the existence of a longitudinal groove along each sister chromatid. The centromere-specific histone H3 variant, CENH3, colocalized with this groove and with microtubule attachment sites. The terminal chromosomal regions were CENH3-negative. During metaphase to anaphase transition, L. elegans chromosomes typically curved to a sickle-like shape, a process that is likely to be influenced by the pulling forces of microtubules along the holocentric axis towards the corresponding microtubule organizing regions. A single pair of 45S rDNA sites, situated distal to Arabidopsis-telomere repeats, was observed at the terminal region of one chromosome pair. We suggest that the 45S rDNA position in distal centromere-free regions could be required to ensure chromosome stability. Copyright (C) 2011 S. Karger AG, Basel
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