Journal
CYTOGENETIC AND GENOME RESEARCH
Volume 125, Issue 4, Pages 286-291Publisher
KARGER
DOI: 10.1159/000235935
Keywords
B chromosomes; Chromosome painting; Eyprepocnemis plorans; Grasshopper; Microdissection
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Funding
- Spanish Ministerio de Ciencia y Tecnologia [CGL2006-06307, CGL2006-05308/BOS]
- Plan Andaluz de Investigacion [P06-CVI-1664, BIO 165 and BIO 220]
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The relative location of 2 repetitive DNAs, i.e. ribosomal (rDNA) and a tandemly repeated satellite DNA (satDNA), with respect to the centromere, suggested that B chromosomes in the grasshopper Eyprepocnemis plorans derived intraspecifically from the X chromosome. To test this hypothesis, we microdissected X and B chromosomes and amplified the obtained DNA by 2 different procedures, the conventional DOP-PCR method and the single-cell whole-genome amplification GenomePlex (R) method. We then generated DNA probes to perform chromosome painting. Our results have confirmed that X and B chromosomes share many DNA sequences between them and with most of the autosomes, especially at locations where the satDNA and rDNA reside, in consistency with previous information. This supports the hypothesis of an intraspecific origin of B chromosomes in E. plorans. Nevertheless, the present results did not help to clarify whether Bs were derived from the X chromosome or else from 1 or more autosomes. Copyright (C) 2009 S. Karger AG, Basel
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