Glycosite Analysis in Glycoproteomics by Mass Spectrometry

Glycoproteomic researches have been rapidly progressing in the last few years. Although intact glycopeptide analysis which obtains glycosite and corresponding glycan information simultaneously is still challenging, various studies, combining glycosylation enrichment methods and mass spectrometry techniques, have accumulated a considerable amount of protein glycosite data for either N- or O-glycosylation or O-GlcNAcylation. This review will majorly focus on recent research outputs in the field of glycosite exploration.