Journal
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
Volume 9, Issue 5, Pages 362-367Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/138920108785915166
Keywords
Full-length cDNA; EST; SAGE; LongSAGE; SuperSAGE; transcriptome
Funding
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG002600] Funding Source: NIH RePORTER
- NHGRI NIH HHS [R01 HG002600] Funding Source: Medline
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The multiple levels of post-transcriptional processing and million-fold differences in expression levels make fully decoding the transcriptome in any given species extremely challenging. This review addresses the influence of sequenced length on transcriptome decoding under current DNA sequencing capabilities. Comparison of full-length cDNA, EST, 14-base SAGE, 21-LongSAGE, 26-SuperSAGE, and other variations shows that the sequenced length has been a key factor in determining the sensitivity and specificity for mRNA detection.
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