Analysis of Organic Components Released from Dental Resin Composites in Saliva and Other Biological Fluids Using Chromatographic Techniques

Dental composites are tooth filling materials. Their chemical composition is complex since they are composed of synthetic polymers, ceramic reinforcing fillers, molecules, which promote or modify the polymerization reaction that yields the cross-linked polymer matrix from the dimethacrylate resin monomers, and silane coupling agents, which enable bonding of the reinforcing fillers to the polymer matrix. Each of these components is critical for a successful dental restoration. Residual monomers in the resin matrix such as triethylene glycol dimethacrylate (TEGDMA), Bis-phenol A glycidyl dimethacrylate (BIS-GMA), and urethane dimethacrylate (UDMA), additives and degradation products from resin-based dental restorative materials eluted into the oral cavity may influence the biocompatibility of these materials. The determination of compounds eluted from dental materials in biological fluids is of utmost importance and requires reliable quantification methods useful for studies addressing cytotoxic, genotoxic and estrogenic potential of these substances. In this review recent chromatographic methods developed for the determination: i.e. quantification and identification of components released from dental composites in saliva and other biological fluids will be presented.