4.5 Article

CryoEM of bacterial secretion systems

Journal

CURRENT OPINION IN STRUCTURAL BIOLOGY
Volume 52, Issue -, Pages 64-70

Publisher

CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2018.08.007

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Funding

  1. European Research Council
  2. Swiss National Science Foundation
  3. Gebert Ruf Foundation
  4. Helmut Horten Foundation

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The need for bacteria to interact with their environment has driven the evolution of elaborate secretion systems. By virtue of their function, secretion systems are macromolecular complexes associated with the cell envelope and therefore inherently difficult to study by conventional structural biology techniques. Cryo-electron microscopy (cryoEM) has become an invaluable technique to study large membrane-embedded complexes and led to major advances in the mechanistic understanding of secretion systems. CryoEM comprises of two main modalities, namely single particle analysis and tomography. Here, we review how detailed structures retrieved by single particle analysis combine elegantly with tomography experiments in which the secretion systems are observed in their native cellular context.

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