Journal
CURRENT OPINION IN STRUCTURAL BIOLOGY
Volume 23, Issue 5, Pages 725-733Publisher
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2013.06.008
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Funding
- NIH [5R01 EY005216]
- JSEI [2T32EY007026-36A1]
- Jules Stein Professor Endowment
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Molecular flexibility over a wide time range is of central importance to the function of many proteins, both soluble and membrane. Revealing the modes of flexibility, their amplitudes, and time scales under physiological conditions is the challenge for spectroscopic methods, one of which is site-directed spin labeling EPR (SDSL-EPR). Here we provide an overview of some recent technological advances in SDSL-EPR related to investigation of structure, structural heterogeneity, and dynamics of proteins. These include new classes of spin labels, advances in measurement of long range distances and distance distributions, methods for identifying backbone and conformational fluctuations, and new strategies for determining the kinetics of protein motion.
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