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Transient expressions of synthetic biology in plants

Journal

CURRENT OPINION IN PLANT BIOLOGY
Volume 19, Issue -, Pages 1-7

Publisher

CURRENT BIOLOGY LTD
DOI: 10.1016/j.pbi.2014.02.003

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Funding

  1. UK Biotechnological and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant 'Understanding and Exploiting Plant and Microbial Secondary Metabolism' [BB/J004596/1]
  2. John Innes Foundation
  3. University of Queensland Postdoctoral Fellowship
  4. BBSRC [BB/L014130/1] Funding Source: UKRI
  5. Biotechnology and Biological Sciences Research Council [BB/L014130/1, BBS/E/J/00000166] Funding Source: researchfish

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Recent developments in transient expression methods have enabled the efficient delivery and expression of multiple genes within the same plant cell over a timescale of days. In some cases, the vectors deployed can be fine-tuned to allow differential expression of the various genes. This has opened the way to the deployment of transient expression for such applications as the production of macromolecular complexes and the analysis and manipulation of metabolic pathways. The ability to observe the effect of gene expression in a matter of days means that transient expression is becoming the method of choice for many plant-based synthetic biology applications.

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