Pervasive regulation of nucleoid structure and function by nucleoid-associated proteins

Bacterial DNA is organised in a compact nucleoid body that is tightly associated with the coupled transcription and translation of mRNAs. This structure contains abundant DNA-binding proteins which perform both structural and regulatory roles, and, in Escherichia coli, serve to buffer and organise pervasive DNA superhelicity. We argue that NAPs coordinate regulation of gene expression and superhelicity at the global (or chromosomal) and at local (corresponding to promoter activity and genetic recombination) levels.