Journal
JOURNAL OF NEUROSCIENCE
Volume 35, Issue 10, Pages 4418-4426Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3741-14.2015
Keywords
circuit mapping; dual-channel stimulation; motor cortex; optogenetics; ReaChR; somatosensory cortex
Categories
Funding
- Howard Hughes Medical Institute
- National Institutes of Health [NS027177]
Ask authors/readers for more resources
Cortical cells integrate synaptic input from multiple sources, but how these different inputs are distributed across individual neurons is largely unknown. Differences in input might account for diverse responses in neighboring neurons during behavior. We present a strategy for comparing the strengths of multiple types of input onto the same neuron. We developed methods for independent dual-channel photostimulation of synaptic inputs using ChR2 together with ReaChR, a red-shifted channelrhodopsin. We used dual-channel photostimulation to probe convergence of sensory information in the mouse primary motor cortex. Input from somatosensory cortex and thalamus converges in individual neurons. Similarly, inputs from distinct somatotopic regions of the somatosensory cortex are integrated at the level of single motor cortex neurons. We next developed a ReaChR transgenic mouse under the control of both Flp- and Cre-recombinases that is an effective tool for circuit mapping. Our approach to dual-channel photostimulation enables quantitative comparison of the strengths of multiple pathways across all length scales of the brain.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available