Journal
CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 46, Issue -, Pages 156-163Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2018.07.019
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Funding
- Emmy Noether program of the German Research Foundation (DFG) [CO82212-1]
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The genetic incorporation of non-canonical amino acids (ncAAs) equipped with photo-crosslinking and chemical crosslinking moieties has found broad application in the study of protein protein interactions from a unique perspective in live cells. We highlight here applications of photo-activatable ncAAs to map protein interaction surfaces and to capture protein-protein interactions, and we describe recent efforts to efficiently couple photo-crosslinking with mass spectrometric analysis. In addition, we describe recent advances in the development and application of ncAAs for chemical crosslinking, including protein stapling, photo-control of protein conformation, two-dimensional crosslinking, and stabilization of transient and low-affinity protein-protein interactions. We expect that the field will keep growing in the near future and enable the tackling of ambitious biological questions.
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