Journal
CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 46, Issue -, Pages 99-107Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2018.07.011
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Funding
- National Science Foundation Graduate Research Fellowship
- National Institutes of Health [GM112728, HD085206]
- National Science Foundation [CBET-1603930]
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In nature, biological processes are regulated with precise spatial and temporal resolution at the molecular, cellular, and organismal levels. In order to perturb and manipulate these processes, optically controlled chemical tools have been developed and applied in living systems. The use of light as an external trigger provides spatial and temporal control with minimal adverse effects. Incorporation of light-responsive amino acids into proteins in cells and organisms with an expanded genetic code has enabled the precise activation/deactivation of numerous, diverse proteins, such as kinases, nucleases, proteases, and polymerases. Using unnatural amino acids to generate light-triggered proteins enables a rational engineering approach that is based on mechanistic and/or structural information. This review focuses on the most recent developments in the field, including technological advances and biological applications.
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