4.5 Review

Target deconvolution techniques in modern phenotypic profiling

Journal

CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 17, Issue 1, Pages 118-126

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2012.12.022

Keywords

-

Funding

  1. NIH [R01 EB005011, R01 AI078947]
  2. Sungshin Women's University Research Grant [2012-2-21-003/1]

Ask authors/readers for more resources

The past decade has seen rapid growth in the use of diverse compound libraries in classical phenotypic screens to identify modulators of a given process. The subsequent process of identifying the molecular targets of active hits, also called 'target deconvolution', is an essential step for understanding compound mechanism of action and for using the identified hits as tools for further dissection of a given biological process. Recent advances in 'omics' technologies, coupled with in silico approaches and the reduced cost of whole genome sequencing, have greatly improved the workflow of target deconvolution and have contributed to a renaissance of 'modern' phenotypic profiling. In this review, we will outline how both new and old techniques are being used in the difficult process of target identification and validation as well as discuss some of the ongoing challenges remaining for phenotypic screening.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available