Journal
CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 17, Issue 5, Pages 854-859Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2013.07.017
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Funding
- Swedish Research Council
- European Research Council
- Knut and Alice Wallenberg Foundation
- USA National Institutes of Health
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Single-cell imaging mass spectrometry (IMS) is a powerful technique used to map the distributions of endogenous biomolecules with subcellular resolution. Currently, secondary ion mass spectrometry is the predominant technique for single-cell IMS, thanks to its submicron lateral resolution and surface sensitivity. However, recent methodological and technological developments aimed at improving the spatial resolution of matrix assisted laser desorption ionization (MALDI) have made this technique a potential platform of single-cell IMS. MALDI opens the field of single-cell IMS to new possibilities, including single cell proteomic imaging and atmospheric pressure analyses; however, sensitivity is a challenge. In this report, we estimate the availability of proteins and lipids in a single cell and discuss strategies employed to improve sensitivity at the single-cell level.
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