Journal
CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 17, Issue 2, Pages 215-220Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2013.02.022
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Funding
- German Research Foundation (DFG) [Bo1862/6-1]
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Protein engineering is the most important method to overcome the limitations of natural enzymes as biocatalysts. The past few years have seen a tremendous increase in novel concepts to facilitate the design of mutant libraries for focused directed evolution mostly guided by advanced bioinformatic tools. In addition, advanced high-throughput methods were developed using, for example, FACS analysis or microfluidic systems. These achievements significantly facilitate the tailor-made design of enzymes to make them suitable for industrial applications.
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