Journal
CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 17, Issue 5, Pages 809-817Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2013.06.019
Keywords
-
Categories
Funding
- National Science Foundation (CAREER Award)
- American Society for Mass Spectrometry
- Oak Ridge Associated Universities
- University of Michigan
- University of Michigan Rackham Graduate School
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1253384] Funding Source: National Science Foundation
Ask authors/readers for more resources
Mass spectrometry (MS) plays a number of key roles in the discovery and development phases for modern pharmaceutical compounds, ranging from the assessment of protein-ligand binding to biomarker discovery. Historically, however, MS has had a relatively limited role in the drug discovery process in comparison to high-throughput fluorescence and radiometric screens. This picture may be changing, however, as many presumptive protein targets are coupled to human disease pathways through specific protein-protein interactions and protein conformations, rather than enzyme activities. This fact will likely drive the development of high-throughput analytical tools that put a stronger emphasis on the structural information content produced in a screen. Here we summarize recent developments surrounding ion mobility-mass spectrometry (IM-MS), one such MS-based tool that is capable of rapidly measuring changes in protein structure, oligomeric state, and binding stoichiometry from complex mixtures at relatively low concentrations.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available