Journal
CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 15, Issue 1, Pages 144-148Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2010.11.004
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Funding
- NIBIB NIH HHS [P30 EB009998, P30 EB009998-01] Funding Source: Medline
- NIGMS NIH HHS [U54 GM074945-01] Funding Source: Medline
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About one-third of all proteins are associated with a metal. Metalloproteomics is defined as the structural and functional characterization of metalloproteins on a genome-wide scale. The methodologies utilized in metalloproteomics, including both forward (bottom-up) and reverse (top-down) technologies, to provide information on the identity, quantity, and function of metalloproteins are discussed. Important techniques frequently employed in metalloproteomics include classical proteomic tools such as mass spectrometry and 2D gels, immobilized-metal affinity chromatography, bioinformatic sequence analysis and homology modeling, X-ray absorption spectroscopy and other synchrotron radiation based tools. Combinative applications of these techniques provide a powerful approach to understand the function of metalloproteins.
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