Journal
CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 14, Issue 3, Pages 383-389Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2010.02.020
Keywords
-
Categories
Funding
- National University of Singapore (NUS) [R-143-000-353-123]
- POSCO TJ Park Foundation
Ask authors/readers for more resources
Diversity-oriented fluorescence library approaches have significantly accelerated the development of new sensors. By making use of combinatorial chemistry and high-throughput screening, they can circumvent our limitations in designing probes for particular recognition processes. Combinatorial chemists have proved how to derivatize fluorogenic scaffolds, tune their photophysical spectra and adjust their properties (from cell permeability to quantum yields) to generate libraries of potential sensors. Several platforms (in vitro assays, cell-based imaging) have also been optimized to screen these libraries in a high-throughput manner, and with the recent progress in image acquisition and analysis, their scope has been expanded toward more diverse and demanding biological systems. Supported by successful examples of fluorescent sensors for biomolecules, proteins, or even phenotypes, this review (together with a video abstract) stresses the important role that diversity-oriented approaches will continue to play in probe development in the near future.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available