Journal
CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 12, Issue 2, Pages 151-158Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2008.01.027
Keywords
-
Categories
Ask authors/readers for more resources
We address recent developments in the area of laboratory, or directed evolution, with a focus on enzymes and on new methodologies of generic potential. We survey three main areas: (i) library making techniques, including the application of computational and rational methods for library design; (ii) screening and selection techniques, including recent applications of enzyme screening by FAGS (fluorescence activated cell sorter); (iii) new approaches for performing directed evolution, and in particular, the application of 'neutral drifts' (libraries generated by rounds of mutation and selection for the enzyme's original function) and of consensus mutations to generate highly evolvable starting points for directed evolution.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available