Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 30, Issue -, Pages 32-39Publisher
CURRENT BIOLOGY LTD
DOI: 10.1016/j.copbio.2014.04.011
Keywords
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Funding
- Joint BioEnergy Institute - Office of Science, Office of Biological and Environmental Research of the U.S. Department of Energy [DE-AC02-05CH11231]
- National Science Foundation [EEC-0540879]
- Department of Energy, ARPA-E Electrofuels Program [DE-0000206-1577]
- National Science Foundation Graduate Research Fellowship Program [DGE 1106400]
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Engineering modular polyketide synthases (PKSs) has the potential to be an effective methodology to produce existing and novel chemicals. However, this potential has only just begun to be realized. We propose the adoption of an iterative design-build-test-learn paradigm to improve PKS engineering. We suggest methods to improve engineered PKS design by learning from laboratory-based selection; adoption of DNA design software and automation to build constructs and libraries more easily; tools for the expression of engineered proteins in a variety of heterologous hosts; and mass spectrometry-based high-throughput screening methods. Finally, lessons learned during iterations of the design-build-test-learn cycle can serve as a knowledge base for the development of a single retrosynthesis algorithm usable by both PKS experts and non-experts alike.
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