Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 22, Issue 3, Pages 465-472Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2011.02.010
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This article summarizes general design principles for functional metagenomics. The focus is on Escherichia coli as an expression host, although alternative host-vector systems are discussed in relation to optimizing gene recovery in activity-based screens. Examples of DNA isolation and enrichment approaches, library construction and phenotypic read-out are described with special emphasis on the use of high throughput technologies for rapid isolation of environmental clones encoding phenotypic traits of interest.
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