Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 21, Issue 5, Pages 640-645Publisher
CURRENT BIOLOGY LTD
DOI: 10.1016/j.copbio.2010.07.003
Keywords
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Funding
- Association Francaise contre les Myopathies
- Vaincre la Mucoviscidose
- Ligue Nationale contre le Cancer
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Chemical vectors for non-viral gene delivery are based on engineered DNA nanoparticles produced with various range of macromolecules suitable to mimic some viral functions required for gene transfer. Many efforts have been undertaken these past years to identify cellular barriers that have to be passed for this issue. Here, we summarize the current status of knowledge on the uptake mechanism of DNA nanoparticles made with polymers and liposomes, their endosomal escape, cytosolic diffusion, and nuclear import of pDNA. Studies reported these past years regarding pDNA nanoparticles endocytosis indicated that there is no clear evident relationship between the ways of entry and the transfection efficiency. By contrast, the sequestration of pDNA in intracellular vesicles and the low number of pDNA close to the nuclear envelop are identified as the major intracellular barriers. So, intensive investigations to increase the cytosolic delivery of pDNA and its migration toward nuclear pores make sense to bring the transfection efficiency closer to that of viruses.
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